We will refer the cells activated with pmaionomycinanticd3 activation itreg cell. Bioassay record aid submitted burnham center for chemical genomics uhts luminescence assay for the identification chemical inhibitors tcell specific antigen receptorinduced nfkb activation. Induced after coaddition phorbol 12myristate 13acetate pma and ionomycin. Incubate the cells for hours pmaionomycin stimulation phaionomycin stimulation hours anticd3cd28 stimulation peptide stimulation 37. Ionomycin able activate and pr